High oil content rape can increase oil production per unit area because it was widely concerned. But the difference of metabolites in different oil content rape seeds was not clear. In this research, the metabolites of two near isogenic lines of Brassica napus with different oil content were analyzed by uplc-q-TOF/MS. The results showed that 46 kinds of differential metabolites were screened out, among which 24 were related to the pathway of oil synthesis, 14 to the pathway of fatty acid synthesis, 3 to the pathway of sugar metabolism, 2 to the pathway of vitamin metabolism, 1 to the pathway of hormone metabolism, and 2 to the pathway of anthocyanin metabolism. The fatty acids such as sterol, anthocyanin, stearic acid and linoleic acid were higher and linolenic acid was lower. The results were helpful to reveal the mechanism of oil synthesis in rape seeds and provide reference for high oil content rapeseed breeding.