Research Report

Transcriptome Profiling of Lisianthus (Eustoma grandiflorum) under Drought Stress  

Xia An1 , Qiang Zhu1 , Xuping Lou2 , Lufeng Li2 , Jie Chen3 , Tingting Liu1 , Wenlue Li1 , Xiahong Luo1 , Guanlin Zhu1 , Lijun Yu1
1 Zhejiang Xiaoshan Institute of Cotton & Bast Fiber Crops Research, Zhejiang Institute of Landscape Plants and Flowers, Hangzhou, 311251, China
2 Hangzhou Xiaoshan District Agricultural Science and Technology Research Institute, Hangzhou, 311202, China
3 Huazhong Agricultural University, Wuhan, 430070, China
Author    Correspondence author
Molecular Plant Breeding, 2020, Vol. 11, No. 31   doi: 10.5376/mpb.2020.11.0031
Received: 27 Oct., 2020    Accepted: 18 Nov., 2020    Published: 26 Dec., 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

An X., Zhu Q., Lou X.P., Li L.F., Chen J., Liu T.T., Li W.L., Luo X.H., Zhu G.L., and Yu L.J., 2020, Transcriptome profiling of Lisianthus (Eustoma grandiflorum) under drought stress, Molecular Plant Breeding, 11(31): 1-7 (doi: 10.5376/mpb.2020.11.0031)

Abstract

Lisianthus (Eustoma grandiflorum) is an important ornamental plant native to North America. So far, the number of basic molecular research reports on Lisianthus is relatively small, especially the research related to the molecular mechanism of response to drought stress. In this experiment, transcriptome second-generation sequencing technology was used to study Lisianthus seedlings under drought stress. The results showed that the transcriptome sequencing data had good quality control results. In order to obtain genetic information on this basis, the sequences were spliced using software such as Trinity and Corset, a total of 102,014 non-redundant genes were obtained, including 2,929 coding genes which were found to belong to 79 transcription factor families after analyzing. Annotating all genes, Zhongguo coffee was found to be the species with the highest comparison similarity. Finally, simple sequence repeat (SSR) analysis was performed on the gene sequences, and all SSR information contained in non-redundant genes and transcription factor coding genes had been obtained. The results of this study can provide candidate molecular resources for subsequent studies on Lisianthus 's response to drought stress in the future.

Keywords
Lisianthus; Drought stress; Transcriptome
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. Xia An
. Qiang Zhu
. Xuping Lou
. Lufeng Li
. Jie Chen
. Tingting Liu
. Wenlue Li
. Xiahong Luo
. Guanlin Zhu
. Lijun Yu
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