Construction of Plant Regeneration System of Glycyrrhiza mongolicum and Glycyrrhiza Xinjiang
College of Life Sciences, Yan\'an University, Yan\'an, 716000, China
Molecular Plant Breeding, 2021, Vol. 12, No. 3
Received: 02 Dec., 2020 Accepted: 19 Jan., 2021 Published: 29 Jan., 2021
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In order to establish the plant regeneration system of Glycyrrhiza mongolicum and Glycyrrhiza Xinjiang, this study used hypocotyls, leaves, cotyledons and stems to inducing callus and regenerated plant, and it screen out the most suitable explants and the best medium for plant regeneration. The experimental results showed that MS+2.0 mg/L NAA+2.0 mg/L 6-BA was the best medium for callus induction and plant regeneration. Among these, hypocotyls and cotyledons are the best explants for callus induction. The tissue induction rate is higher than 90%. The rapid propagation system established in this study can provide the theoretical reference and practical guidance for the breeding and production of fine Glycyrrhiza uralensis Fisch. varieties.
Glycyrrhiza uralensis Fisch.; Sterile seedlings; Callus; Regenerated plants
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