Development and Application of SSR Makers with Transcriptional Sequencing Data of Female and Male flowers in Chestnut
Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Fruit and Tea Subcenter of Hubei Innovation Center of Agricultural Science and Technology, Wuhan 430064
Plant Gene and Trait, 2020, Vol. 11, No. 2
Received: 27 Apr., 2020 Accepted: 28 Apr., 2020 Published: 28 Apr., 2020
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This article was first published in Molecular Plant Breeding ( ISSN1672-416X，CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In present study, nineteen simple sequence repeat (SSR) loci were isolated and characterized from male and female flower-specific expression genes, which derived from a transcriptional sequencing database of female and male flowers in chestnut. Among these pairs of SSR primers, five pairs showed good polymorphism and productive stability by PCR analysis. Seventeen chestnut cultivars were used to perform SSR-PCR experiments and the PCR products were determined with capillary electrophoresis (CE) method. The results showed that seventeen cultivars were divided into three groups. The first group included six cultivars such as ‘Chaoduanzhi 1’, ‘Chaoduanzhi 2’, ‘Yimeng Duanzhi’ and ‘Yanhong’, which belongs to northern chestnut varieties. The second group contained ten cultivars, which were mainly southern chestnut varieties composing of some local varieties of Hubei province. The last group contained only one cultivar ‘Jinliwang’, which was separately different relationship from other cultivars in the first and second groups. Taken together, these results indicated that the SSR markers exhibited good repeatability and high polymorphism. Our study provided novel insights into analyzing genetic diversity and developing specific molecular marker assisted with male and female flower of chestnut.
Chestnut; Transcriptional sequencing; SSR; Capillary electrophoresis
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