Research Report

Characterization of Genomic Microsatellite Markers and Analysis of Pollen Donors Number for Single Cone of Chinese fir  

Kuipeng Li1 , Shichang Chen1 , Leiming Dong2 , Ji Liang3 , Daixi Chen1 , Kaiyong Huang1
1 Guangxi Forestry Research Institute, Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China/Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Nanning, 530002, China
2 Chinese Academy of Forestry, Research Institute of Forestry, Beijing, 100091, China
3 Guangxi University, Forestry College, Nanning, 530004, China)
Author    Correspondence author
Tree Genetics and Molecular Breeding, 2020, Vol. 10, No. 5   doi: 10.5376/tgmb.2020.10.0005
Received: 29 May, 2020    Accepted: 12 Aug., 2020    Published: 14 Aug., 2020
© 2020 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Li K.P., Chen S.C., Dong L.M., Liang J., Chen D.X., and Huang K.Y., 2020, Characterization of genomic microsatellite markers and analysis of pollen donors number for single cone of Chinese fir, Tree Genetics and Molecular Breeding, 10(5): 1-7 (doi: 10.5376/tgmb.2020.10.0005)


Chinese fir [Cunninghamia lanceolate (Lamb.) Hook] is one of the most important indigenous timber tree species in China. The aim of the present work is to characterize simple sequence repeat (SSR) loci derived from the specific length amplified fragment sequencing (SLAF) data of the genome and to investigate the number of pollen donors for per cone was with novel SSR markers of low-frequency null alleles. A total of 58855 SLAF-SSR with frequency of 42.04 SSR/Mbp were identified in about 1.40 Gb Chinese fir genome. Dinucleotide repeat SSR contributed to 66.4% of the total SSR from SLAF data. The AT/AT and ATG/CAT motifs were predominant in the category of din- and trinucleotide repeat SSR. Low frequencies of null alleles (<5%) were detected at the nine novel SSR markers with average expected heterozygosity of 0.513 and polymorphism information content score of 0.508. The number of tested progeny of a cone was from 4 to 13. It could be 67 pollinizers for 15 cones and the average number of pollen donors per cone was 4.5. The study points out, for the first time, that there are multiple pollen donors for single cone in gymnosperm.

Chinese fir; SLAF; SSR; Null alleles; Sibship relationships
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