Identification and Mapping of a Blast Disease Resistance Gene in Rice Line IR65482
1 College of Life Sciences, Fujian Agricultural & Forestry University, Fuzhou, 350002, China
2 Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China
Molecular Plant Breeding, 2019, Vol. 10, No. 5
Received: 01 Jan., 1970 Accepted: 01 Jan., 1970 Published: 18 Mar., 2019
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This article was first published in Molecular Plant Breeding (2018, 13: 4308-4313) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Rice line IR65482 has broad-spectrum resistance to Magnaporthe oryzae from different areas, and it has been known that there is a blast resistance gene Pi40 (t) on its chromosome 6. In the present study, using a bulked segregant analysis sequencing strategy, we identified another rice blast resistance gene on chromosome 11 of IR65482. The F2 population derived from a cross between IR65482 and Nipponbare was further used for gene mapping, and the IR65482 blast resistance gene was mapped in a 425 kb region between InDel markers OSL3-2 and OSL3-5 at the end of chromosome 11. The results could provide guidance for application of IR65482 in breeding rice varieties with blast resistance and theoretical reference for the cloning of IR65482 blast resistance gene in the future.
Rice blast disease; Resistance gene; Genetic analysis; BSA-Seq; Gene mapping
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