Cloning and Expression Analysis of MeTCP4 Transcription Factor from Cassava and Construction of Plant Expression Vector
1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Agriculture Sciences, Haikou, 571101, China
2 College of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China
Molecular Plant Breeding, 2019, Vol. 10, No. 5
Received: 21 Jan., 2019 Accepted: 20 Feb., 2019 Published: 31 May, 2019
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This article was first published in Molecular Plant Breeding (2018, 05: 1517-1523) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The TCP transcription factor family genes are involved in multiple regulation of plant growth and environmental stress. However, these family genes are rarely studied in cassava (Manihot esculenta). There are 36 TCP genes in cassava. Phylogenetic tree analysis indicates that MeTCP can be divided into 8 subgroups. MeTCP4 is one of 36 genes in cassava TCP family, which can be transcribed by miR319 and sheared. MeTCP4 contained an open reading frame (ORF) of 1 269 bp, which encoded 422 amino acids with a predicted molecular weight of 45.75 kD and theoretical PI of 6.17. The full-length cDNA sequence of MeTCP4 was cloned from the cassava genome. The results of quantitative RT-PCR showed that the expression of MeTCP4 can be found in all the tested tissues, with the lowest in roots, second in stems and the highest in leaves. MeTCP4 gene was repressed by drought and low temperature stress. Construction of high and low plant expression vector would provide basis for further study of the function of the gene.
Cassava (Manihot esculenta); Gene clone; MeTCP4 transcription factor; qRT-PCR; Expression vector construction