Principle and Strategy of DNA Fingerprint Identification of Plant Variety
1 Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China
2 Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China
3 Beijing TodaySoft Inc., Beijing, 100083, China
4 Institute of Cotton Research of CAAS, Anyang, 455000, China
5 National Agricultural Technical Extension and Service Center, Beijing, 100125, China
* These authors contributed equally to this work
Molecular Plant Breeding, 2019, Vol. 10, No. 11 doi: 10.5376/mpb.2019.10.0011
Received: 08 Jun., 2019 Accepted: 28 Jul., 2019 Published: 11 Aug., 2019
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This is an open access article published under the terms of the Creative Commons Attribution License
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Preferred citation for this article:
Wang F.G., Tian H.L, Yi H.M., Zhao H., Huo Y.X., Kuang M., Zhang L.K., Lv Y.D., Ding M.Q., and Zhao J.R., 2019, Principle and strategy of dna fingerprint identification of plant variety, Molecular Plant Breeding, 10(11): 81-92 (doi: 10.5376/mpb.2019.10.0011)
Plant variety identification has profound meanings to ensure seed quality and food safety. DNA fingerprinting has its advantage in plant variety identification, and has transformed from indirect evidence into a mainstream method. A method for DNA fingerprinting called core loci combination was summarized in this study, which utilized a set of fixed core loci combination to identify different varieties. Since 2003, the project has been constantly improved and expanded. The first was to form combination of expanded loci by increasing the quantity of core loci to cope with increasing demand of variety identification of derived varieties. The second was to further decompose the core loci into group specific loci which played the function of fast and accurate clustering and species-specific loci which had stronger variety identification function. The third was to further propose the core loci combination of polyploidy crops based on the mature experience of diploid crop, which are diploidized single marker method and combination marker method. From the trend of future development, the rapid development of new technologies such as sequencing technology and gene editing technology will largely promote the improvement of DNA fingerprinting and improve the application effect in practice. This article has systematically elaborated the concept and characteristics of variety identification, summarized major types and identification methods of DNA fingerprinting, extracted technical methods for variety DNA fingerprinting and finally out-looked the future trend of DNA fingerprinting development. It is hoped that the study could provide guidances for the development of DNA fingerprinting standard of each crops, and the application of molecular technology in the structure and variety identification of DNA fingerprinting database.
Plant; Cultivar identification; DNA fingerprinting; Core loci; Extended loci