Xueni Di¹ , Mengsheng Deng¹ , Xue Zou^1,2 , Yanan Li^1,3 , Su Ni^1,3 , Xiyao Wang^1,3

1 College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, China
2 Mianyang Academy of Agricultural Sciences, Mianyang, 621023, China
3 National Demonstration Center for Experimental Crop Science Education, Sichuan Agricultural University, Chengdu, 611130, China
Author    Correspondence author
Molecular Plant Breeding, 2019, Vol. 10, No. 16   doi: 10.5376/mpb.2019.10.0016
Received: 26 Aug., 2019    Accepted: 15 Oct., 2019    Published: 28 Oct., 2019

This article was first published in Molecular Plant Breeding (2018, 16(13): 4143-4150) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Di X.D., Deng M.S., Zou X., Li Y.N., Ni S., and Wang X.Y, 2019, The response analysis of BR related genes at low temperature in potato sprout regulation, 10(16): 121-127 (doi: 10.5376/mpb.2019.10.0016)

Brassinosteroids (BR) plays an important role in regulating plant growth. We found that the expression of BR synthesis and signal transduction genes were up-regulation when potato dormancy was released, and suckering agents can inhibit the expression of Methylsterol monooxygenase 1 (SMO1), Delta24-sterol reductase 1 (DWF1), Protein BR insensitive 1 (BRI1), BR-signaling kinase (BSK) and Cyclin D3 (CYCD3) genes in potato tuber. Besides, we found that the storage period among different cultivars are significant different at low temperature. Low temperature can obviously extend cultivar ‘Favorita’ storage time than cultivar ‘Mira’. In order to explore the response of BR genes to potato sprout at low temperature. This study will analyze the expression of BR synthesis, signal transduction and regulation genes at from cultivar ‘Favorita’ and cultivar ‘Mira’ which both cultivars are storage at low temperature (4°C) and room temperature (23±2)°C environment by qRT-PCR technique. The results showed that the expression of DWF1 and BRI1 are decreased in both cultivars by low temperature treatment. The expression of SMO1, BSK and CYCD3 genes are decreased in cultivar ‘Favorita’by low temperature treatment. While, the expression of SMO1, BSK and CYCD3 genes in‘Mira’are increase by temperature treatment. It shows that low temperature influences the expression of SMO1, BSK and CYCD3 genes in cultivar ‘Favorita’, which results in the long dormancy period of the cultivar ‘Favorita’. These results will provide the theoretical basis in elucidating the BR mechanism of potato sprout, breeding variety selection and new technology research and development of potato storage regulation.

Potato; Dormancy; Sprout; Low temperature; Brassinosteroids