Agrobacterium-mediated Transient Expression of Foreign Gene in Arabidopsis thaliana
The School of Life Science, Jilin Normal University, Siping, 136000
Molecular Plant Breeding, 2020, Vol. 11, No. 13
Received: 25 Jun., 2020 Accepted: 28 Jun., 2020 Published: 10 Jul., 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Viral vector-mediated inoculation is a normal method of transient expression of foreign gene in the plants. In this study, we transiently expressed the foreign gene GUS in Arabidopsis thaliana by using Agrobacterium-mediated leaf-infection and detected the expression of GUS gene after ago-inoculation by GUS staining method and semi-quantitative RT-PCR. The results showed that GUS was expressed on 7-15 day after inoculation in Arabidopsis plants. Moreover，we successfully obtained fast expression of the viral suppressor HCPro in Arabidopsis plants, providing a new system for the fast expression of foreign gene and the study of gene function in plants.
Arabidopsis thaliana ; leaf-infection; foreign gene; Agrobacterium