Transciptome and Metabolomics Analysis of Pepper (Yin Chuan Cavel ) Male Sterility Lind
1 Institute of Germplasm Resources, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan Ningxia 750002
2 General Station of Forest Pest Management and Quarantine of Ningxia, Yinchuan Ningxia, 750001
Molecular Plant Breeding, 2020, Vol. 11, No. 24
Received: 25 Sep., 2020 Accepted: 27 Sep., 2020 Published: 20 Oct., 2020
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This article was first published in Molecular Plant Breeding ( ISSN1672-416X，CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In order to study the relation of different gene expression and Metabolomics difference of pepper (Yin Chuan Cavel )male sterility lind pollen abortion.The transciptiome was sequenced using RNA-seq technology, the metabolomics was tested by UPLC-MS/Ms.The result was analyzed by GO categories and KEGG enrichment analysis.A total of 3319 diferent express
ion genes were successfully obained, include up genes 800, down genes 2519. Of the 536 metabolites identified, a total of 102 metabolites were significantly differentiated .The differential metabolites involved up regulation metabolites
68,down regulation metabolites 34. Included 17 amino acid derivatives, 16 Nucleotides and derivatives, 13 glyceryl ester ,10 sphingolipids ，8 flavonoids and phenolic acids, 6 in other classes (mainly related to carbohydrate metabolism). Differential metabolites and significant differential expression genes are mainly concentrated in protein related amino acid metabolism pathway and flavonoid metabolism pathway. The results showed that the significant differential expression of transcription genes, through the regulation of metabolism related enzymes, resulted in the differential metabolism of lipid, Phenolic acids and carbohydrate metabolites, resulted in pollen abortion of the Pepper (Yin Chuan Cavel ) male sterile.
Pepper; Male sterility; Transciptome; Metabolomics
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