Bioinformatics Analysis of Cellulose Synthase CesA Gene from Miscanthus lutarioriparius
1 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128
2 Miscanthus Ecological Application Technology, Hunan Engineering Laboratory, Changsha 410128
Molecular Plant Breeding, 2021, Vol. 12, No. 1 doi: 10.5376/mpb.2021.12.0001
Received: 03 Jan., 2021 Accepted: 15 Jan., 2021 Published: 22 Jan., 2021
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Li Y., He Y.C., Lin Y.H., Wan T., Li M., and Chen Z.Y., 2021, Bioinformatics analysis of cellulose synthase CesA gene from Miscanthus lutarioriparius, Molecular Plant Breeding, 12(1): 1-8 (doi:10.5376/mpb.2021.12.0001)
Based on 3 generations transcriptome sequencing technology-PacBio SMRT single-molecule real-time sequencing method, supplemented with 2 generation sequencing method, a complete and accurate unigene library was constructed. After comparing with closely related species, the cellulose synthase gene (CesA) 4, 7, 9 gene sequences of Miscanthus lutarioriparius were spliced and named as MlCesA4, MlCesA7 and MlCesA9. Then, Bioinformatics analysis software was used to construct phylogenetic trees of proteins, to predict and analyze the phosphorylation sites of post-translational modifications of amino acids, and to predict and analyze conserved domains of proteins.The analysis results showed that the sequence lengths of MlCesA4, 7, 9 genes were 2980 bp, 3310 bp, and 3208 bp, respectively, which were closely related to Sorghum bicolor and Zea mays; cellulose synthase of Miscanthus lutarioriparius (MlCesA) MlCesA4 and MlCesA9 have 43 phosphorylation sites, MlCesA7 has 48 phosphorylation sites; MlCesA4 is an unstable protein with 6 transmembrane domains, 4 domains are outside the membrane and 3 are inside the membrane.; MlCesA7 and MlCesA9 are stable proteins, both of which have 8 transmembrane domains, 5 domains are outside the membrane and 4 are inside the membrane. The three are all hydrophilic proteins, and the secondary structure is dominated by α-helix and random coils.
Miscanthus lutarioriparius; Cellulose synthase gene (CesA); Bioinformatics analysis