Research Report

Genetic Diversity and Relationship Analysis of 21 Walnut Varieties Based on SSR Markers  

Lingwen Xu1,2 , Yi Wang2 , Xiaolong Yuan2 , Bin Lu2 , Jiabo Hao2 , Yu Zhang2 , Jun Ma3 , Fei Wang3
1 College of Forestry, Southwest Forestry University, Kunming, 650224, China
2 Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry and Grassland and The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration, Kunming, Yunnan, 650201, China
3 Kunming Forestry Technology Promotion Centre, Kunming, 650033, China
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 9   doi: 10.5376/mpb.2021.12.0009
Received: 18 Feb., 2021    Accepted: 17 Mar., 2021    Published: 31 Mar., 2021
© 2021 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Xu L.W., Wang Y., Yuan X.L., Lu B., Hao J.B., Zhang Y., Ma J., and Wang F., 2021, Genetic diversity and relationship analysis of 21 walnut varieties based on SSR markers, Molecular Plant Breeding, 12(9): 1-7 (doi: 10.5376/mpb.2021.12.0009)


In order to grasp the germplasm resources of walnut in Yunnan province, the genetic diversity and relationship of 21 walnuts varieties commonly planted in Yunnan province were analyzed using SSR fluorescent labeling technology. The results showed that the average number of allelic loci of 12 pairs of primers used in 21 walnut species samples was 7.916 7. The mean number of alleles, Shannon Wiener index (I) and polymorphic information content (PIC) per locus were 4.3516, 1.643 6 and 0.705 5. Twelve pairs of polymorphism information content (PIC) were higher than 0.25, indicating that the 12 pairs of primers had good polymorphism in the samples. In the phylogenetic analysis, the 21 walnut varieties were divided into 7 subgroups, indicating that the 21 walnut varieties had rich genetic diversity, extensive gene exchange and complex genetic background in the breeding process.

Walnut; SSR; Genetic analysis; Clustering analysis
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. Yi Wang
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