Mingxin Luo¹ , Fengmin Liu² , Weili Zhang^1,3 , Juntao Cui^3,4 , Donghui Li³ , Jiyan Chen^1,3 , Zhangyong Dong¹

1 College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China
2 Management Center of Teaching and Research Base, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China
3 Deqing Zhongkai Agricultural Tech. Innovation Research Co. Ltd., Zhaoqing, 526642, China
4 College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 20   doi: 10.5376/mpb.2021.12.0020
Received: 15 Jun., 2021    Accepted: 23 Jun., 2021    Published: 30 Jun., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Luo M.X., Liu F.M., Zhang W.L., Cui J.T., Li D.H., Chen J.Y., and Dong Z.Y., 2021, Application of SRAP and CDDP markers in genetic diversity analysis of Morinda officinalis How., Molecular Plant Breeding, 12(20): 1-11 (doi: 10.5376/mpb.2021.12.0020)

The analysis of the main germ plasm resources of Morinda officinalis How. in Zhaoqing area and the definition of the relationship among the varieties will provide scientific basis for the cultivation, protection and identification of Morinda officinalis How.. SRAP and CDDP molecular markers were used to analyze the genetic diversity of 9 Morinda officinalis How. materials in Zhaoqing. The genetic similarity coefficients among samples were calculated by NTSYS-pc2.1 software, cluster analysis was carried out according to UPGMA method, and the results of two kinds of molecular markers were compared and analyzed. 28 pairs of SRAP primers and 16 CDDP primers were used to amplify 140 and 83 bands respectively, of which the number of polymorphic bands was 68 and 63 respectively, the polymorphism ratio (PPB) was 48.6% and 75.9% respectively, the genetic similarity coefficient varied from 0.69 to 0.96 and 0.53 to 0.95, and the average genetic similarity coefficient was 0.83 and 0.69 respectively. When the genetic similarity coefficient of SRAP was 0.86 and that of CDDP was 0.71, the four varieties of Guangning special type, Guangning special grafting type, local large leaf type and local lobular type were grouped into one group. SRAP and CDDP molecular markers can be used for genetic diversity analysis of Morinda officinalis How.. There are some differences between SRAP and CDDP, so the combined analysis of the two kinds of markers can more accurately reveal the genetic relationship between Morinda officinalis How. germplasm. The results of CDDP clustering were more consistent with those of combined analysis, indicating that CDDP was more suitable for genetic diversity analysis of Morinda officinalis How. than SRAP.

Morinda officinalis How.; SRAP; CDDP; Genetic diversity; Cluster analysis