Using QTL-seq to mine genes related to petiole length of cabbage
Xiang Tai 1
Xiaowei Zhu 1
Daguo Gu 2
Tianyue Bo 1
1 Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106
2 Shanghai Xinghui Vegetables Company Limited, Shanghai, 201419
Plant Gene and Trait, 2020, Vol. 11, No. 1
Received: 26 Apr., 2020 Accepted: 26 Apr., 2020 Published: 26 Apr., 2020
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This article was first published in Molecular Plant Breeding ( ISSN1672-416X，CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The length of the petiole determines the area of a single plant. It is great significance to mining genes related to the length of the petiole of cabbage. In this experiment, short-petiole of high-bred line 301 (male parent)、long-petiole of high-bred line 294 (female parent) and its F2 were used as materials. Extreme trait materials were selected from F2 (30 long-petiole and 30 short-petiole materials) were used to construct pools of DNA. The candidate genes related to the petiole length of cabbage were mining using combination of QTL-seq and SNP / InDel-index. In this study, six candidate genes controlling the petiole length were identified from genome of cabbage. Seven candidate genes were enriched in the three pathways of SKU5 protein coding, (XTH) coding and plant cell wall synthesis. This study provides a reference for cloning of genes related to petiole length in cabbage.
Cabbage; Length of the petiole; QTL-seq
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