Cloning and Expression Analysis of CoSAUR32 and CoSAUR50 Genes in Camellia oleifera
Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education; Key Lab of Non-wood Forest Products of State Forestry Administration; Cooperative Innovation Center of Cultivation and Utilization for Nonwood Forest Trees of Hunan Province; Central South University of Forestry and Technology, Changsha, Hunan, 410004
Tree Genetics and Molecular Breeding, 2020, Vol. 10, No. 2
Received: 12 May, 2020 Accepted: 14 May, 2020 Published: 14 May, 2020
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This article was first published in Molecular Plant Breeding ( ISSN1672-416X，CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this study, Camellia oleifera ‘Huashuo’ was used as the experimental material. Two Samall auxin-up RNAs SAUR family genes were cloned by PCR and named CoSAUR32 and CoSAUR50. CoSAUR32 gene open reading frame (ORF) is 372 bp, encoding 123 amino acids, CoSAUR50 gene open reading frame (ORF) is 318 bp, encoding 105 amino acids. Sequence analysis showed that CoSAUR32 and CoSAUR50 proteins have no signal peptide and transmembrane structure, and can be expressed in chloroplasts and nucleus. Homology comparison and phylogenetic tree analysis showed that the two SAURs in Camellia oleifera have the closest relationship to the SAUR in Camellia sinensis. The results of RT-qPCR showed that the expression of CoSAUR32 and CoSAUR50 genes the expression level in self-pollination ovary was significantly higher than that in cross-pollination ovary at 36 h, and in self-pollination styles was significantly higher than that in cross-pollination styles at 24 h. It is speculated that CoSAUR32 and CoSAUR50 participate in the process of self-incompatibility. This study provides a reference for exploring the function of SAUR genes and analyzing of the molecular mechanism of self-incompatibility of Camellia oleifera.
Camellia oleifera; CoSAUR32; CoSAUR50; Gene cloning; Gene expression
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Tree Genetics and Molecular Breeding
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