Validation of housekeeping genes as internal control for studying gene expression in Rhododendron Molle G. Don Located on Dabie Mountains
Hubei Collaborative Innovation Center for the CharacteristicResources Exploitation of Dabie Mountains, College of Life Science, Huanggang Normal University, Huanggang, 438000
Tree Genetics and Molecular Breeding, 2020, Vol. 10, No. 3
Received: 25 Jul., 2020 Accepted: 27 Jul., 2020 Published: 27 Jul., 2020
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This article was first published in Molecular Plant Breeding ( ISSN1672-416X，CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Rhododendron molle G. Don, a member of the family of Rhododendron, possesses high ornamental and medicinal value. As a species specific to Dabie Mountains, its distribution was scattered. The estimation of expression stability of internal reference genes was vital for molecular biological and genetic research. In this study, expression pattern of candidate genes RmRPL3-1, RmNAC-1, RmCOX1-4, RmRmPDS-1, RmUBQ-6, and RmL10-4 were analyzed in different tissues of R. molle by real-time quantitative RT-PCR, including root, stem, leaf, and flower. According to GeNorm, NormFinder, and Best-keepersoftwares, the most stable internal reference in different tissues of R. molle was the combination of RmCOX1-4 and RmUBQ-6. The most stable internal reference genes selected in this study will provide a basis for the following gene expression study in R. molle.
Rhododendron molle G. Don; Reference genes; qRT-PCR; expression pattern
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Tree Genetics and Molecular Breeding
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