A Preliminary Study on Somatic Cell Embryogenesis of Quercus mongolica
Shenyang Agricultural University Forestry College
Tree Genetics and Molecular Breeding, 2020, Vol. 10, No. 6 doi: 10.5376/tgmb.2020.10.0006
Received: 18 Nov., 2020 Accepted: 20 Nov., 2020 Published: 04 Dec., 2020
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Preferred citation for this article:
Jin Z.H., Li Q., Li M.Y., Liu C.P., and Lu X.J., 2020, A preliminary study on somatic cell embryogenesis of Quercus mongolica, Tree Genetics and Molecular Breeding, 10(6): 1-8 (doi:10.5376/tgmb.2020.10.0006)
In order to establish a callus induction and somatic embryogenesis system of Quercus mongolica, and provide an effective culture system for molecular mechanism research of somatic embryogenesis, the leaves of annual seedlings of Quercus mongolica were used as explants to study the main influencing factors of callus induction and somatic embryogenesis. The results show that the best way to disinfect the leaves was as follows: 75% alcohol 30 s + 0.1% HgCl2 4 min; The best medium combination for callus induction is: MS+0.5 mg/L 2,4-D+0.5 mg/L 6-BA, the induction rate is 96.7%; The best medium combination for somatic embryogenesis was MS + 0.1 mg/L 2,4-D + 1 mg/L 6-BA, the induction rate was 58.0%. In this study, the best way of callus induction from Quercus mongolica leaves was selected and spherical embryos were induced, which laid a foundation for further research on the establishment of somatic embryogenesis system of Quercus mongolica.
Quercus mongolica; Somatic embryogenesis; Callus; Leaf
Tree Genetics and Molecular Breeding
• Volume 10