lu yefei , Kewei Ou , Qing Luo , Yu Zhang , Qin Cheng , Pengjin Zhu , Quanguang Zhou , Xinhua Pang , Ping Lv

Guangxi Subtropical Crops Research Institute, Nanning, 530001
Author    Correspondence author
Molecular Plant Breeding, 2020, Vol. 11, No. 9   
Received: 16 Jun., 2020    Accepted: 16 Jun., 2020    Published: 16 Jun., 2020

This article was first published in Molecular Plant Breeding ( ISSN1672-416X，CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Sugarcane 'GR2' and 'GL05136' belong to late and early maturing high sugar varieties. Transcriptome sequencing was performed on the mixed samples of the leaves and stems of the two varieties to compare the differences in gene expression and to screen and clone genes that may be involved in sugar synthesis. Two comparison methods of interspecific at the same period and intraspecific at different periods were adopted, and four comparison groups were set up in the two varieties. KEGG and GO databases were used to analyze the overall transcriptome and the gene differential expression in the related fields of sugar synthesis. The results showed that there were 97 gene sequences of 5 enzymes related to sugar synthesis and several high-expression genes whose functions were unknown. Subsequently, the function of the high-expression genes could be verified by building a transgenic sugarcane validation system, improving the molecular mechanism theory of sugar metabolism, developing high-sugar molecular markers and even building high-expression transgenic varieties.

Sugarcane; Sugar synthesis; Transcriptome; ‘GR2’; ‘GL05136’