Over-expression of  JcSEUSS1  from  Jatropha curcas  Induces the Accumulation of Anthocyanin in Leaves

Jingxian Wang<sup>1,2</sup>; Xin Ming<sup>1,2</sup>; Yanbin Tao<sup>2</sup>; Mingyong Tang<sup>2</sup>; Zengfu Xu<sup>2</sup>

Research Report

Over-expression of JcSEUSS1 from Jatropha curcas Induces the Accumulation of Anthocyanin in Leaves

Jingxian Wang^1,2

, Xin Ming^1,2

, Yanbin Tao²

, Mingyong Tang²

, Zengfu Xu²

1 School of Life Sciences, University of Science and Technology of China, Hefei, 230027
2 Key Laboratory of Tropical Plant Resources and Sustainable Use, Innovative Academy of Seed Design, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, 666303

Author

Correspondence author
Molecular Plant Breeding, 2020, Vol. 11, No. 23
Received: 06 Sep., 2020 Accepted: 09 Sep., 2020 Published: 09 Sep., 2020

This article was first published in Molecular Plant Breeding ( ISSN1672-416X，CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Anthocyanins are a class of important secondary metabolites in plants and play important roles in photoprotection and antioxidation. In this study, we cloned the JcSEUSS1 gene from Jatropha curcas. Sequence alignment indicates JcSEUSS1 contains a highly conserved LIM domain, and the sequence of JcSEUSS1 is similar to other SEUSS proteins. The expression pattern of JcSEUSS1 in different organs of Jatropha plants was analyzed by real-time fluorescence quantitative PCR, and it was found that the gene was expressed in all tissues. Furthermore, JcSEUSS1 over-expression significantly promoted the accumulation of anthocyanin in leaves of Jatropha. The expression of chalketone synthase (JcCHS) and chalketone isomerase (JcCHI), which are involved in anthocyanin biosynthesis, were significantly up-regulated in JcSEUSS1-transgenic lines. Our results indicate that JcSEUSS1 may positively regulate the anthocyanin synthesis in leaves of Jatropha.

Keywords

Jatropha curcas; JcSEUSS1; Over-expression; Anthocyanin

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