Research Report

Mapping of Nuclear Male-Sterile Gene ms14 Using SSR Markers in Cotton  

Peizheng Wang , Lei Bian , Cong Cao
Hainan Tropical Ocean University, Sanya, Hainan, 572022, China
Author    Correspondence author
Molecular Plant Breeding, 2016, Vol. 7, No. 35   doi: 10.5376/mpb.2016.07.0035
Received: 25 Oct., 2016    Accepted: 14 Dec., 2016    Published: 23 Dec., 2016
© 2016 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wang P.Z., Bian L., and Cao C., 2016, Mapping of nuclear male-sterile gene ms14 using SSR markers in cotton, Molecular Plant Breeding, 7(35): 1-5 (doi: 10.5376/mpb.2016.07.0035)

Abstract

Nuclear male sterility (NMS) is a very important character for cotton breeding and genetics programs. Identification of linked molecular markers with NMS will greatly facilitate breeding for this trait. Among all of the Nuclear male-sterile genes, only Dong-A (ms14) was utilized successfully in hybrid production of cotton. Dong-A (ms14), a recessive NMS line developed from spontaneous mutation in upland cotton, has been applied most widely and played an increasing role in hybrid cultivar development in China. An interspecific F2 population comprised of 180 individual plants was developed by crossing a cultivar of Kang A (Dong-A derived Lines, Gossypium hirsutum L.) to a cultivar of 601588 (G. barbadense L.). Basing on this population the ms14 gene was mapped on chromosome 2 and closely linkaged with BNL3971 marker within a genetic distance of 16.7 cM. The BNL3971 marker could be used for the marker assisted selection in breeding a new cultivar line, and provide the information for closely location and further gene isolation by map based cloning.

Keywords
Cotton; Molecular mapping; Nuclear male-sterile genes; ms14
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