Yang Li ^1,2* , Yuxiang Yuan^2* , Yanyan Zhao² , Xiaochun Wei² , Qiuju Yao² , Wusheng Jiang² , Zhiyong Wang² , Shuangjuan Yang² , Xiaowei  Zhang² , Baoming Tian¹

1 College of Life Science, Zhengzhou University, Zhengzhou, Henan, China
2 Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, China
* These authors contributed equally to this work
Author    Correspondence author
Molecular Plant Breeding, 2017, Vol. 8, No. 5   doi: 10.5376/mpb.2017.08.0005
Received: 28 Feb., 2017    Accepted: 13 Jun., 2017    Published: 28 Jul., 2017

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Li Y., Yuan Y.X., Zhao Y.Y., Wei X.C., Yao Q.J., Jiang W.S., Wang Z.Y., Yang S.J., Zhang X.W., and Tian B.M., 2017, Pathogen identification of clubroot disease in Chinese cabbage from Yuanyang county, Henan province, Molecular Plant Breeding, 8(5): 45-51 (doi: 10.5376/mpb.2017.08.0005)

To clarify the causal agent of clubroot disease in Chinese cabbage from Yuanyang experimental base, the isolate YY was collected from the clubroot for further research. After morphological, cytological observation and pathogenicity identification, the pathogen was preliminary inferred as Plasmodiophora brassicae. In order to quickly detect the pathogen, specific primers were designed according to D85819 and Pro1 gene, respectively, and used to amplify the expected fragment from the template DNA of target pathogen. After PCR and sequence analysis, they showed 99% and 93% identity with the D85819 and Pro1 gene sequences of P. brassicae from NCBI respectively. These results indicated that the pathogen was finally confirmed as P. brassicae, which were coincident with preliminary speculation. Moreover, Pro1-specific marker developed in this study can be applied to quick and accurate detection of P. brassicae isolate, which appears a useful assay method for detecting P. brassicae resting spores and improves efforts to halt the dissemination and better control this pathogen.

Yuanyang County; Clubroot disease; Chinese cabbage; Pathogen indentification