Yunwei Cheng , Wei Deng , Yelei Lu , Shaopeng Han , Yang Lv , Gongjian Zeng , Chao Zhou , Dechun Zhang , Xiangling Shen

Key Laboratory of Three Gorges Regional Plant Genetics & Germplasm Enhancement (CTGU)/ Biotechnology Research Center, China Three Gorges University, Yichang, 443002
Author    Correspondence author
Molecular Plant Breeding, 2020, Vol. 11, No. 5   doi: 10.5376/mpb.2020.11.0005
Received: 08 Jun., 2020    Accepted: 09 Jun., 2020    Published: 09 Jun., 2020

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cheng Y.W., Deng W., Lu Y.L., Han S.P., Lv Y., Zeng G.J., Zhou C., Zhang D.C., and Shen X.L., 2020, Establishment of sorghum BTx623 immature embryos genetic transformation and regeneration system, Molecular Plant Breeding, 11(5): 1-8 (doi: 10.5376/mpb.2020.11.0005)

Sorghum is one of the world's important crops after wheat, rice, maize, and barley. Although the sorghum genome had been well-sequenced, genetic breeding and functional genome research in sorghum cultivar BTx623 is still limited due to the lack of efficient and stable genetic transformation and regeneration system in sequencing. In this study, the immature embryos of sorghum genome-sequencing cultivar BTx623 was used as the explants material, and the bar gene resistant to phosphoglyphosate was used as the screening marker for Agrobacterium-mediated sorghum genetic transformation. By screening the adaptability of callus to different concentrations of phosphoglyphosate, the appropriate concentration of phosphoglyphosate in the genetic transformation of sorghum cultivar BTx623 was determined to be 2.5 mg/L, and BTx623 immature embryo was used as explants to obtain resistant callus. After screening, regenerated plants were obtained by treating resistant callus with 0.0067 mg/L ZNC in regeneration medium. Therefore, this study successfully obtained resistant callus and regenerating plants, and established a genetic transformation and regeneration system in sorghum cultivar BTx623, which may have great significance for functional genome research and genetic breeding in sorghum.

Sorghum bicolor; BTx623; Immature embryo; Agrobacterium-transformation; Regeneration