In order to study the relation of different gene expression and Metabolomics difference of pepper (Yin Chuan Cavel) male sterility lind pollen abortion. The transciptiome was sequenced using RNA-seq technology, the metabolomics was tested by UPLC-MS/Ms. The result was analyzed by GO categories and KEGG enrichment analysis. A total of 3 319 different expression genes were successfully obained, include up genes 800, down genes 2 519. Of the 536 metabolites identified, a total of 102 metabolites were significantly differentiated. The differential metabolites involved up regulation metabolites 68, regulation metabolites 34. Included 17 amino acid derivatives, 16 Nucleotides and derivatives, 13 glyceryl ester, 10 sphingolipids, 8 flavonoids and phenolic acids, 6 in other classes (mainly related to carbohydrate metabolism). Differential metabolites and significant differential expression genes are mainly concentrated in protein related amino acid metabolism pathway and flavonoid metabolism pathway. The results showed that the significant differential expression of transcription genes, through the regulation of metabolism related enzymes, resulted in the differential metabolism of lipid, Phenolic acids and carbohydrate metabolites, resulted in pollen abortion of the Pepper (Yin Chuan Cavel) male sterile.