Zhenxiang He^1,2 , Minghuai Wang³ , Xiuyu Xu³ , Yunfei You⁴ , Yong Chen⁵ , Liangbo Zhang⁶

1 Life Sciences College, Nanjing University, Nanjing, 210023, P.R. China
2 Nanjing University (Suzhou) High-tech Institute, Suzhou, 215123, P.R. China
3 Guangdong Academy of Forestry, Guangzhou, 510520, P.R. China
4 Yangkou National Forest Farm, Shunchang, 353211, P.R. China
5 Xishan Forest Farm, Chongqing, 404100, P.R. China
6 Hunan Academy of Forestry, Changsha, 410004, P.R. China
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 5   doi: 10.5376/mpb.2021.12.0005
Received: 12 Jan., 2021    Accepted: 29 Jan., 2021    Published: 07 Apr., 2021

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

He Z.X., Wang M.H., Xu X.Y., You Y.F., Chen Y., and Zhang L.B., 2021, The development of EST-SSR markers from transcriptome sequencing and genetic diversity of twenty genotypes with high yields of Cornus wilsoniana, an important wood oil plant, Molecular Plant Breeding, 12(5): 1-10 (doi:10.5376/mpb.2021.12.0005)

Oil plants are not only used as essential nutriments in food but also used broadly as ingredients of industrial products. Cornus wilsoniana Wanger is an important native woody oil plant with high-yield and high-oiliness characters in China. The fruit oil of Cornus wilsoniana not only could be used as equilibrated dietary oil, but also has hypolipidemic function and helps to overcome EFA deficiency. Genomic information is currently not available for Cornus wilsoniana, which will therefore affect its genetic improvement process. In this study, 8713 EST-SSRs were identified from the transcriptome sequencing of Cornus wilsoniana. Most of these EST-SSRs were composed of dimer and trimer repeats. The AG/CT motif is the most common dimeric EST-SSRs motif (53.5%), whereas the CG/CG (0.44%) microsatellites are present only at low abundances. Among the trimeric microsatellites, AAG/CTT (5.53%) and ATC/ATG (4.27%) are the most common motifs. There are no obvious dominant motifs among the tetra-, penta-, and hexa- nucleotide motifs. Fifteen pairs of EST-SSRs primers are developed with polymorphism and used for the genetic diversity analysis of twenty genotypes of Cornus wilsoniana with high fruit output. Allele number per locus ranges from 2 to6 with an average of 2.87, and the PIC value ranges from 0.05 to 0.58 with an average of 0.36. Average genetic diversity overall SSR loci for the 20 genotypes was 0.427, ranging from 0.049 to 0.659. All the loci are polymorphic and clearly distinguish the genotypes. Cluster analyze (NJ tree, UPGMA) identifies a similar pattern of variation. 15developed EST-SSRs are informative, codominant and reliable, and could be applied in the improvement programs of Cornus wilsoniana in the future.

Woody oil plant; Genetic diversity; Polymorphism; Cornus wilsoniana