Yuqiu Liao¹ , Fangfang Wang¹ , Xi Zhu² , Ning Zhang¹ , Huaijun Si^1,3

1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
2 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
3 Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 11   doi: 10.5376/mpb.2021.12.0011
Received: 19 Mar., 2021    Accepted: 22 Mar., 2021    Published: 31 Mar., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Liao Y.Q., Wang F.F., Zhu X., Zhang N., and Si H.J., 2021, Screening and bioinformatics analysis of proteins interacting with StMAPKK1 in potato by yeast two-hybrid system, Molecular Plant Breeding, 12(11): 1-9 (doi: 10.5376/mpb.2021.12.0011)

Mitogen-activated protein kinase (MAPK) cascade reaction is one of important and complex signal networks involved in plant growth and development, hormone and stress response. As one of its main members, mitogen-activated protein kinase kinase (MAPKK) is located in the middle of the cascade reaction and plays a key role in signal collection and divergence. There were shown that potato (Solanum tuberosum L.) StMAPKK1 (PGSC0003DMT400000744) gene responded to drought stress. Therefore, StMAPKK1 gene was firstly selected to screen its interacting protein. In this study, the bait vector pGBKT7-StMAPKK1 was constructed by homologous recombination and used to screen potato cDNA library by yeast (Saccharomyces cerevisiae) two-hybrid system. Five StMAPKK1-interacting proteins, hydrolase (hydrolyzing O-glycosyl compound), RING-H2 subgroup RHE protein, cyanate hydratase, ARF GTPase activator, and a C2 domain-containing protein, were obtained through this screening and were identificated by bioinformatics analysis, and the interaction was verified by small-scale hybridization verification. The results provided theoretical basis for further study on the signal pathway and biological function of potato StMAPKK1.

Potato; StMAPKK1; Yeast two-hybrid; Interacting protein