Danqing Yao¹ , Jianming Xia¹ , Jianfeng Lou¹ , Qinqin Gu¹ , Jian Liu¹ , Yu Zhang¹ , Qiuli Zhang¹ , Weiwei Zhang²

1 Shanghai Agricultural Technology Extension and Service Center, Shanghai, 201103, China
2 Shanghai Vocational College of Agriculture and Forestry, Shanghai, 201699, China
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 25   doi: 10.5376/mpb.2021.12.0025
Received: 19 Apr., 2021    Accepted: 25 Aug., 2021    Published: 03 Sep., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Yao D.Q., Xia J.M., Lou J.F., Gu Q.Q., Liu J., Zhang Y., Zhang Q.L., and Zhang W.W., 2021, Identification of seed purity on hybrid japonica rice ‘Huayou 14’ using SSR markers, Molecular Plant Breeding, 12(25): 1-7 (doi: 10.5376/mpb.2021.12.0025)

In this study, the hybrid japonica rice ‘Huayou 14’ and its parents were used as materials, using SSR molecular marker technology, to select primers and identify seed purity according to its polymorphism. From 48 pairs of candidate SSR molecular markers widely distributed on 12 rice chromosomes, 6 pairs of primers were screened, which showed stable codominance between the hybrid and its parents. Completely distinguished, using these primers to carry out molecular purity identification of ‘Huayou 14’ seeds, and comparing the identification results with the field planting purity identification results, the results showed that the identification results of the two were basically the same. Finally, the RM274 and RM1195 marker combinations with the most polymorphic sites and the most significant differences in specificity were finally determined as core primers, and a more rapid and effective technical method for identifying the seed purity of hybrid japonica rice ‘Huayou 14’ was established.

SSR marker; Hybrid japonica rice; ‘Huayou 14’; Purity identification