Chen Liang^* , Yajie Liu^* , Li Wang , Guoqiang Du , Xiaoxin Shi

College of Horticulture, Hebei Agricultural University, Baoding, 071000, China
*These authors contributed equally to this work
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 35   doi: 10.5376/mpb.2021.12.0035
Received: 28 Oct., 2021    Accepted: 04 Nov., 2021    Published: 12 Nov., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Liang C., Liu Y.J., Wang L., Du G.Q., and Shi X.X., 2021, Telomerase activity of apple plantlets in Vitro kept relative stable after conserved for years, Molecular Plant Breeding, 12(35): 1-8 (doi:10.5376/mpb.2021.12.0035)

In order to understand the telomerase characteristics in relation to the proliferation and aging of the tissue culture plantlets of apple, the leaves, stem segments and callus sampled from Malus domestica ‘Golden Delicious’, Malus domestica ‘Fuji’ and Malus domestica ‘Gala’ plantlets that subcultured in vitro for years were analyzed for the telomerase activity and the amount of telomerase reverse transcriptase gene expression by using modified TRAP and Quantitative Real-time PCR. The results showed that the telomerase activity in the leaves and stem segments had no significant difference among the plantlets that subcultured for different times. During a given subculture time, the telomerase activity in leaves showed a tendency of increasing first and then declining along with the leaf aging in a ninety-day period. The telomerase activity varied in different materials, among which Malus domestica ‘Golden Delicious’ callus and Malus domestica ‘Gala’ leaves appeared the highest amount, respectively. The expression of telomerase reverse transcriptase gene was consistent with the change of telomerase activity.

Apple; Plantlet in vitro; Subcultured times; Plantlet aging; Telomerase activity; Telomerase reverse transcriptase