Shuangshuang Wang , Gang Zeng , Quanli Dou , Bolin Zhang , Qin Tian

College of Biological and Agricultural Science and Technology, Zunyi Normal University, Zunyi, 563006, China
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 38   doi: 10.5376/mpb.2021.12.0038
Received: 23 Dec., 2021    Accepted: 26 Dec., 2021    Published: 31 Dec., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wang S.S., Zeng G., Dou Q.L., Zhang B.L., and Tian Q., 2021, Cloning and tissue expression analysis of calcium-dependent protein kinase gene BsCDPK1 in Bletilla striata, Molecular Plant Breeding, 12(38): 1-9 (doi: 10.5376/mpb.2021.12.0038)

Calcium-dependent protein kinases (CDPKs) play vital roles in the regulation of plant growth, development, and the response to adversity stress. Studying the sequence information and expression pattern of CDPK gene could lay the foundation for the protein structure, gene functions and signal network of plant stress resistance. The full-length cDNA of BsCDPK1 gene was obtained by RT-PCR and RACE approaches in this study. The physicochemical properties and structural domains of BsCDPK1 protein were analyzed by bioinformatics tools; In addition, amino acid sequence alignment and phylogenetic analysis of BsCDPK1 were performed. The expression pattern of BsCDPK1 gene in the seedling stage of Bletilla striata was detected by real-time fluorescence quantitative PCR. The result showed that the full cDNA of the cloned BsCDPK1 was 1 981 bp, encoding 496 amino acids. The molecular weight of encoded protein was 55.997 KD, isoelectric point 5.38, aliphatic index 87.50 and instability index 43.54. The BsCDPK1 protein included the typical kinase domain of CDPKs in other plants, autoinhibitory domain, ATP binding site and the four EF-hands were highly conserved, which was more homologous with CDPKs of Dendrobium candidum and Phalaenopsis equestris, clustered on the same evolutionary branch. The results of qRT-PCR indicated that BsCDPK1 gene was expressed in the roots, stems and leaves in Bletilla striata seedlings. The cloning and sequence characterizing and tissue expression pattern analysis of BsCDPK1 provided experimental basis for elucidating the function of BsCDPK1 gene in plant growth and development and adversity stress responses.

Bletilla striata; Calcium-dependent protein kinase; Gene cloning; Expression profiling