Jieying Chen , Zhengguo Liu , Chunling Wang , Qingming Chen , Zhikui Cheng , Lianlian Ma , Jiquan Gou

Agricultural College, Guangxi University, Nanning, 530001, P.R. China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 1   doi: 10.5376/mpb.2022.13.0001
Received: 27 Dec., 2021    Accepted: 03 Jan., 2022    Published: 11 Jan., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Chen J.Y., Liu Z.G., Wang C.L., Chen Q.M, Cheng Z.K., Ma L.L., and Gou J.Q., 2022, Genetics of SSR polymorphism marker and purity identification of hybrid seeds in wax gourd (Benincasa hispida), Molecular Plant Breeding, 13(1): 1-6 (doi: 10.5376/mpb.2022.13.0001)

Seed purity identification is an indispensable process before seeds are used in production and is the key to ensuring seed quality. Developing an accurate, effective, inexpensive and convenient method for Wax gourd seeds purity identification can provide technical support for seed quality. The co-dominant primers of XD-18 and XD-99 were chosen to test the seed purity of ‘Jinyuan 2’, ‘Jinyuan 3’ and ‘Lvxianzi 2’ 3 Wax Gourd hybrids in this research. The F₂ generations of Wax Gourd hybrids was used to analyze whether the 2 SSR markers were in accordance with Mendelian separation law. If it is in accordance with Mendelian separation, then 2 pairs of primers were used to carry out SSR molecular marker purity identification of hybrid seeds of Wax gourd, and a comparison experiment was performed between the SSR molecular marker purity identification and the field planting purity identification. The results showed that the 2 SSR markers were in accordance with the Mendelian separation law of 1: 2: 1 in the F₂ generation population and SSR molecular marker purity identification of Wax gourd hybrid seeds was highly consistent with the field planting purity identification. Therefore, this study provides a more accurate technical system for the purity identification of Wax gourd seeds and other crop hybrid seeds.

Wax gourd; SSR marker; Mendelian separation; Hybrid purity