Shuangbin Fu^1,2 , Yanping Yang¹ , Zhuang Zhou¹

1 Zhejiang Institute of Subtropical Crops, Wenzhou, 325005, P.R. China
2 Fujian Ornamental Plant Germplasm Resources Innovation & Engineering Application Research Center, Fuzhou, 350002, P.R. China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 4   doi: 10.5376/mpb.2022.13.0004
Received: 06 Jan., 2022    Accepted: 16 Jan., 2022    Published: 10 Feb., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Fu S.B., Yang Y.P., and Zhou Z., 2022, Induction, regeneration and artificial seed production of etiolation stems of Cymbidium hookerianum, Molecular Plant Breeding, 13(4): 1-8 (doi:10.5376/mpb.2022.13.0004)

The protocorm-like bodies (PLBs) of Cymbidium hookerianum were used as the explants, the effects of different hormones and hormone concentrations on the induction of etiolation stems, the subsequent regeneration of etiolation stems, and artificial seeds production and conservation using etiolation stems were studied. The results showed that the most efficient etiolation stems production induced by PLBs was obtained by adding 0.1 mg/L NAA and 0.1 mg/L 6-BA. The induced etiolation stems cultivated on the medium containing 2.0/0.1 mg/L NAA/6-BA showed the highest induction rate of PLBs, with an average of 80.40%, and the induction number of PLBs was higher, with an average of 1.36. The Induced etiolation shoot tips could regenerate rootless seedlings on the medium, and these seedlings could root and strengthen on the medium of 1/2MS + 20.0 g/L sucrose + 7.5 g/L agar. The highest germination rate of artificial seeds produced by encapsulating etiolation shoot tips was obtained on the MS medium containing 1.0 mg/L 6-BA, with an average of 86.67%. After storing for 6 weeks, the germination rate of artificial seeds preserved at 4°C was the highest, with an average of 48.00%.

Cymbidium hookerianum; Etiolation stem; Artificial seed; Storage