Like Wang , Shanguo Mao , Ping Yang , Huiping Zhong , Yunwen Wu , Ying He , Quanzhan Chen

School of Food Science, Nanjing Xiaozhuang University, Nanjing, 211171, P.R. China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 8   doi: 10.5376/mpb.2022.13.0008
Received: 10 Feb., 2022    Accepted: 17 Feb., 2022    Published: 04 Mar., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wang L.K., Mao S.G., Yang P., Zhong H.P., Wu Y.W., He Y., and Chen Q.Z., 2022, Establishment of rapid propagation system of Lycium Ruthenicum Murr tissue culture, Molecular Plant Breeding, 13(8): 1-5 (doi: 10.5376/mpb.2022.13.0008)

Lycium ruthenicum is a perennial shrub of Lycium in Solanaceae, which is an important plant for improving the environment of desert greening. The sterile seedlings of Lycium ruthenicum were inoculated in callus induction medium MS-1 (MS+0.5~1 mg/L NAA+1.5~2.5 mg/L 6-BA+30 g/L sucrose + 8 g/L agar, pH5.2) for about 10 days. After 40 days, the callus was separated from the explants and placed in MS-2 medium (MS + 0.5～1 mg/L NAA + 0.5~1.4 mg/L 6-BA + 30 g/L sucrose + 6 g/L agar, pH5.2) to induce adventitious buds. Finally, the buds were inoculated in MS medium to take root. Rooting in MS medium about 20 days later, the seedlings grew up, each seedling had 3-4 roots, and then carried out seedling refining and transplanting. This method of callus induction is due to obtaining callus directly from sterile seedlings of Lycium ruthenicum. And it shorts the time of tissue culture, reduces the cost of culture and improves the efficiency of culture in Lycium ruthenicum.

Lycium Ruthenicum Murr; Tissue culture; MS medium