Author Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 8 doi: 10.5376/mpb.2022.13.0008
Received: 10 Feb., 2022 Accepted: 17 Feb., 2022 Published: 04 Mar., 2022
Wang L.K., Mao S.G., Yang P., Zhong H.P., Wu Y.W., He Y., and Chen Q.Z., 2022, Establishment of rapid propagation system of Lycium Ruthenicum Murr tissue culture, Molecular Plant Breeding, 13(8): 1-5 (doi: 10.5376/mpb.2022.13.0008)
Lycium ruthenicum is a perennial shrub of Lycium in Solanaceae, which is an important plant for improving the environment of desert greening. The sterile seedlings of Lycium ruthenicum were inoculated in callus induction medium MS-1 (MS+0.5~1 mg/L NAA+1.5~2.5 mg/L 6-BA+30 g/L sucrose + 8 g/L agar, pH5.2) for about 10 days. After 40 days, the callus was separated from the explants and placed in MS-2 medium (MS + 0.5~1 mg/L NAA + 0.5~1.4 mg/L 6-BA + 30 g/L sucrose + 6 g/L agar, pH5.2) to induce adventitious buds. Finally, the buds were inoculated in MS medium to take root. Rooting in MS medium about 20 days later, the seedlings grew up, each seedling had 3-4 roots, and then carried out seedling refining and transplanting. This method of callus induction is due to obtaining callus directly from sterile seedlings of Lycium ruthenicum. And it shorts the time of tissue culture, reduces the cost of culture and improves the efficiency of culture in Lycium ruthenicum.