Jingxian Wang^1,2 , Xin Ming^1,2 , Yanbin Tao² , Mingyong Tang² , Zengfu Xu²

1 School of Life Sciences, University of Science and Technology of China, Hefei, 230027, China
2 Key Laboratory of Tropical Plant Resources and Sustainable Use, Innovative Academy of Seed Design, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, 666303, China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 10   doi: 10.5376/mpb.2022.13.0010
Received: 24 Mar., 2022    Accepted: 12 Apr., 2022    Published: 02 Jun., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wang J.X., Ming X., Tao Y.B., Tang M.Y., and Xu Z.F., 2022, Over-expression of JcSEUSS1 from Jatropha curcas induces the accumulation of anthocyanin in leaves, Molecular Plant Breeding, 13(10): 1-11 (doi: 10.5376/mpb.2022.13.0010)

Anthocyanins are a class of important secondary metabolites in plants and play important roles in photoprotection and antioxidation. In this study, we cloned the JcSEUSS1 gene from Jatropha curcas. Sequence alignment indicates JcSEUSS1 contains a highly conserved LIM domain, and the sequence of JcSEUSS1 is similar to other SEUSS proteins. The expression pattern of JcSEUSS1 in different organs of Jatropha plants was analyzed by real-time fluorescence quantitative PCR, and it was found that the gene was expressed in all tissues. Furthermore, JcSEUSS1 over-expression significantly promoted the accumulation of anthocyanin in leaves of Jatropha. The expression of chalketone synthase (JcCHS) and chalketone isomerase (JcCHI), which are involved in anthocyanin biosynthesis, were significantly up-regulated in JcSEUSS1-transgenic lines. Our results indicate that JcSEUSS1 may positively regulate the anthocyanin synthesis in leaves of Jatropha.

Jatropha curcas; JcSEUSS1; Over-expression; Anthocyanin