Xin Kong^1* , Jie Liu^1* , Jiyi Gong¹ , Kamran Malik² , Li Wang¹ , Xianlei Chen¹ , Ping Zhang¹ , Jianfeng Wang^2,3 , Yin Yi¹

1 Key Laboratory of National Forestry and Grassland Administration for Biodiversity Conservation in Karst Mountains Areas of Southwestern China, Key Laboratory of Plant Physiology and Developmental Regulation, School of Life Sciences, Guizhou Normal University, Guiyang, 550001, China
2 State Key Laboratory of Grassland Agro-ecosystems; Center for Grassland Microbiome; Collaborative Innovation Center for Western Ecological Safety; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730000, China
3 State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, China
* These authors contributed equally to this work
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 16   doi: 10.5376/mpb.2022.13.0016
Received: 25 Mar., 2022    Accepted: 16 May, 2022    Published: 29 May, 2022

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Kong X., Liu J., Gong J.Y., Malik K., Wang L., Chen X.L., Zhang P., Wang J.F., and Yi Y., 2022, Effects of Erwinia sp. infection on the changes of metabolisms in Dendrobium officinale, Molecular Plant Breeding, 13(16): 1-12 (doi: 10.5376/mpb.2022.13.0016)

The current study was aimed to investigate the changes in metabolites and metabolic pathways in Dendrobium officinale stem infected by Erwinia sp., the causal agent of D. officinale. A total of 176 metabolites were obtained by LC-MS in D. officinale before and after infection. Besides, the KEGG in MetaboAnalyst 5.0 (https://www.metaboanalyst.ca/) was used to analyze the pathways of the metabolites, of which only 73 metabolites were obtained Output ID from KEGG. The alanine, aspartate and glutamate metabolism, phenylalanine metabolism, isoquinoline alkaloid biosynthesis, lysine degradation, lysine biosynthesis, citrate cycle (TCA cycle), cutin, suberine, and wax biosynthesis, pyruvate metabolism, starch, and sucrose metabolism, and glyoxylate and dicarboxylate metabolism were the important metabolic pathways found by pathway enrichment analysis on KEGG ID. Furthermore, based on the information above, using VIP>1.0 and P<0.05 as screening criteria, there were only 68 differential metabolites among 73 metabolites. The metabolic pathways and differential metabolites analysis revealed that the contents of amino acid, organic acid, and nucleotides of EI (D. officinale infected with Erwinia sp.) plant were higher than EF (D. officinale free from Erwinia sp.) plant. Hence, D. officinale can enhance its structure by increasing the organic acids (citric acid, succinic acid, etc.) and amino acids (proline, arginine, etc.) to resist the pathogens.

Dendrobium officinale; Erwinia sp.; LC-MS; Metabolic pathways