Yashu Peng¹ , Luhua Li^1,2 , Yabing Dong¹ , Ruhong Xu^1,2 , Mingjian Ren^1,2

1 College of Agriculture, Guizhou University, Guiyang, 550025, China
2 Guizhou Sub-Center of National Wheat Improvement Center, Guiyang, 550025, China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 11   doi: 10.5376/mpb.2022.13.0011
Received: 12 Apr., 2022    Accepted: 20 Apr., 2022    Published: 02 Jun., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Peng Y.S., Li L.H., Dong Y.B., Xu R.H., and Ren M.J., 2022, Cloning of GzABI5-3A3 gene of ‘Guizimai 1’ and functional analysis in tobacco, Molecular Plant Breeding, 13(11): 1-11 (doi:10.5376/mpb.2022.13.0011)

ABI5 plays an important role in many biological processes such as seed dormancy and germination, growth and development, anthocyanin synthesis and response to stress. A large number of studies have shown that ABI5 is involved in seed dormancy and germination, while few studies have shown that ABI5 is involved in anthocyanin synthesis. In order to explore the involvement of ABI5 transcription factors in the regulation of wheat anthocyanin synthesis, the full cDNA length of GzABI5-3A3 was amplified from a color wheat variety ‘Guizimai 1’. GzABI5-3A3 may be involved in biological processes regulating plant growth, photosynthesis, flowering, seed germination and anthocyanin accumulation. Phylogenetic tree analysis showed that GzABI5-3A3 was homologous to TaABI5D-SH-31, TaABI5D-SH-23 and TaABI5D-SW-23 in wheat. In this study, the overexpression vector of GzABI5-3A3 gene PBI121-GzABI5-3A3 was further constructed for tobacco genetic transformation. Eight tobacco transgenic lines were obtained by genetic transformation. Studies on GzABI5-3A3overexpressed transgenic lines showed that the anthocyanin content in seedling leaves of transgenic tobacco lines L4, L7 and L15 was significantly lower than that of the wild type, and the expression levels of anthocyanin synthesis pathways of structural genes NtPAL, NtDFR, NtANS and NtCHS were significantly decreased. The results showed that GzABI5-3A3 could negatively regulate anthocyanin synthesis by regulating the expression of structural genes in anthocyanin synthesis pathway. This study provides a research basis for the subsequent research on the molecular mechanism of GzABI5-3A3 regulating anthocyanin synthesis.

GzABI5-3A3; Gene clone; Tobacco genetic transformation