Lulu Meng¹ , Guandi He^2,3,4 , Weijun Tian¹ , Dandan Li¹ , Yun Huang¹ , Tengbing He^1,5

1 College of Agriculture, Guizhou University, Guiyang, 550025, China
2 Institute of Agro-Bioengineering of Guizhou University, Guiyang, 550025, China
3 Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, China
4 College of Life Sciences, Guizhou University, Guiyang, 550025, China
5 Institute of New Rural Development of Guizhou University, Guiyang, 550025, China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 13   doi: 10.5376/mpb.2022.13.0013
Received: 25 Apr., 2022    Accepted: 07 May, 2022    Published: 02 Jun., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Meng L.L., He G.D., Tian W.J., Li D.D., Huang Y., and He T.B., 2022, Cloning and expression analysis of NAC2 gene of potato (Solanum tuberosum L), Molecular Plant Breeding, 13(13): 1-9 (doi: 10.5376/mpb.2022.13.0013)

NAC is one of the unique transcription factor families in plants, which plays an important regulatory role in response to abiotic stress. In this study, a NAC transcription factor gene StNAC2 was cloned in potato ‘Yunshu 505’ by RT-PCR. Sequence analysis showed that the ORF of StNAC2 gene was 876 bp, encoding 291 amino acids, including a conserved Nam domain with a molecular weight of 33.585 12 kD, a theoretical isoelectric point of 7.04, an instability coefficient of 50.33 and an average hydrophilic number of -0.660. Protein prediction results showed that StNAC2 protein is an unstable hydrophilic protein with no signal peptide and transmembrane domain, which can be expressed in both cytoplasm and mitochondria. Homology comparison and phylogenetic tree analysis showed that StNAC2 gene is closely related to Capsicum annuum and Nicotiana tabacum. Spatio-temporal expression results showed that the expression of StNAC2 gene was different in tissue, the expression of StNAC2 gene was higher in leaves than in stems. The expression of StNAC2 gene was induced under cadmium stress, and reached the maximum value at 50 mg/kg, but decreased significantly after being treated with 100 mg/kg cadmium, indicating that the expression of StNAC2 gene was inhibited under high concentration stress and StNAC2 gene could involve in cadmium stress. The results lay the foundation for dissecting the molecular mechanism of StNAC2 gene involved in Cd stress response.

Potato (Solanum tuberosum); StNAC2; Transcription factor; Gene cloning; Expression analysis