Xiaojuan Wu¹ , Bobo Fan¹ , Naqin Zhao¹ , Yanfu Chen¹ , Changhai Song² , Zhuo Yu¹ , Yanhong Ma¹

1 Agronomy College, Inner Mongolia Agricultural University, Hohhot, 010019, China
2 Hulun Buir Agr-Tech Extension and Service Center, Hulun Buir, 021306, China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 15   doi: 10.5376/mpb.2022.13.0015
Received: 11 May, 2022    Accepted: 17 May, 2022    Published: 09 Jun., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wu X.J., Fan B.B., Zhao N.Q., Chen Y.F., Song C.H., Yu Z., and Ma Y.H., 2022, Analysis of MYB transcription factor family based on transcriptome sequencing in colored potato, Molecular Plant Breeding, 13(15): 1-10 (doi: 10.5376/mpb.2022.13.0015)

MYB transcription factor family is one of the transcription factors with various functions and numerous quantities, which plays an important role in plant growth and metabolism regulation. In this study, based on the transcriptome sequencing data of color potato tubers, we screened MYB transcription factor genes during the development of color potato, and analyzed its conservative domain and subcellular localization; meanwhile, we analyzed the differential expression of R2R3 MYB transcription factor by TMEV software, and predicted the function of R2R3 MYB transcription factor with significant difference. The results showed that based on the transcriptome sequencing (RNA-Seq) data, 143 transcription factors of MYB family were annotated, selected, and divided into four categories (1R-MYB, R2R3-MYB, 3R-MYB and 4R-MYB) according to their structural characteristics. The result of the subcellular localization demonstrated that 138 MYB transcription factors were located in the nucleus, one MYB transcription factors were located in the cytoplasm, and four MYB transcription factors were located in the cell membrane. Conservative domain analysis showed that the conserved motifs of R2R3-MYB transcription factors are R2 and R3. Colored potato R2R3-MYB transcription factor gene was differentially expressed in different periods, among which 10 were up-regulated and 5 were down-regulated. According to the analysis of GO enrichment, 6 transcription factors of R2R3-MYB related to anthocyanin were annotated. This study will provide a reference for further study on the biological function and metabolic regulation mechanism of MYB gene related to anthocyanin synthesis in color potato.

Colored potato; RNA-seq; MYB family; Expression pattern