Mouliang Li , Xiaoni Zhang , Shengnan Lin , Quanshu Wu , Manzhu Bao , Xiaopeng Fu

College of Horticulture and Forestry Sciences, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 17   doi: 10.5376/mpb.2022.13.0017
Received: 18 May, 2022    Accepted: 27 May, 2022    Published: 09 Jun., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Li M.L., Zhang X.N., Lin S.N., Wu Q.S., Bao M.Z., and Fu X.P., 2022, Genome-wide identification and expression analysis of the R2R3-MYB genes family in Rosa chinensis, Molecular Plant Breeding, 13(17): 1-11 (doi: 10.5376/mpb.2022.13.0017)

Rosa chinensis is a commercially important ornamental plant cultivated worldwide with high ornamental and economic value. The objectives of this study were to analyze the structure, conserved domain and evolution of R2R3-MYB by bioinformatics, besides, it was also to study the expression specificity of family members in different tissues and investigate the biological function of R2R3-MYB genes family in Rosa chinensis. According to the reported R2R3-MYB genes of Arabidopsis thaliana, one hundred and twenty members of R2R3-MYB genes in Rosa chinensis ‘Old blush’ were identified by BLAST and Hmmer tool. Phylogenetic tree indicated that these genes were divided into 34 subgroups. The N-terminal of the amino acid sequence of R2R3-MYB family members contains 2 unequal repeat domains R2 and R3. The results of gene structure analysis showed that the R2R3-MYB family genes contain 1 to 11 exons; 120 genes were located on 7 chromosomes of rose, and 8 tandem repeat events occurred by chromosome mapping analysis. The results of analysis of genome correlation, a total of 16 pairs of R2R3-MYB gene clusters were found. Based on the analysis of gene expression patterns, the results showed that 120 genes were wildly expressed throughout the development of the rose. What’s more, they could be divided into 6 subgroups, and there were different patterns of expression between each subgroup.

Rosa chinensis; R2R3-MYB genes family; Bioinformatics; Expression model