Research Article
Genome-wide Identification and Expression Analysis of the R2R3-MYB Genes Family in Rosa chinensis
Author Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 17 doi: 10.5376/mpb.2022.13.0017
Received: 18 May, 2022 Accepted: 27 May, 2022 Published: 09 Jun., 2022
Li M.L., Zhang X.N., Lin S.N., Wu Q.S., Bao M.Z., and Fu X.P., 2022, Genome-wide identification and expression analysis of the R2R3-MYB genes family in Rosa chinensis, Molecular Plant Breeding, 13(17): 1-11 (doi: 10.5376/mpb.2022.13.0017)
Rosa chinensis is a commercially important ornamental plant cultivated worldwide with high ornamental and economic value. The objectives of this study were to analyze the structure, conserved domain and evolution of R2R3-MYB by bioinformatics, besides, it was also to study the expression specificity of family members in different tissues and investigate the biological function of R2R3-MYB genes family in Rosa chinensis. According to the reported R2R3-MYB genes of Arabidopsis thaliana, one hundred and twenty members of R2R3-MYB genes in Rosa chinensis ‘Old blush’ were identified by BLAST and Hmmer tool. Phylogenetic tree indicated that these genes were divided into 34 subgroups. The N-terminal of the amino acid sequence of R2R3-MYB family members contains 2 unequal repeat domains R2 and R3. The results of gene structure analysis showed that the R2R3-MYB family genes contain 1 to 11 exons; 120 genes were located on 7 chromosomes of rose, and 8 tandem repeat events occurred by chromosome mapping analysis. The results of analysis of genome correlation, a total of 16 pairs of R2R3-MYB gene clusters were found. Based on the analysis of gene expression patterns, the results showed that 120 genes were wildly expressed throughout the development of the rose. What’s more, they could be divided into 6 subgroups, and there were different patterns of expression between each subgroup.