Research Article

Identification And Functional Analysis of 3-Phosphatidylinositol Kinase FAB1/PIKfyve Gene Family in Rice (Oryza sativa)  

Yichun Xu , Wanqi Liang , Canhua Wang , Jing Yu
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 19   doi: 10.5376/mpb.2022.13.0019
Received: 18 May, 2022    Accepted: 25 May, 2022    Published: 10 Jun., 2022
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Xu Y.C., Liang W.Q., Wang C.H., and Yu J., 2022, Identification and functional analysis of 3-phosphatidylinositol kinase FAB1/PIKfyve gene family in rice (Oryza sativa), Molecular Plant Breeding, 13(19): 1-12 (doi: 10.5376/mpb.2022.13.0019)


FAB1/PIKfyve is a key enzyme that catalyzes phosphatidylinositol 3-phosphate (PtdIns3P) to form phosphatidylinositol 3,5-bisphosphate (PtdIns (3,5) P2). Its product PtdIns (3,5) P2 plays an important role in the development of eukaryotic cells. In order to figure out the function of PtdIns (3,5) P2 in rice reproductive development, this study combined bioinformatics and genetics methods to identify the rice FAB1/PIKfyve genes, analyzed the physical and chemical properties, gene structures, conserved domains, phylogenetic tree, cis-acting elements, tissue expression profiles and used CRISPR/Cas9 gene editing technology to obtain osfab1b mutants. Bioinformatics analysis results showed that nine FAB1 gene family members were identified from the whole genome of Oryza Sativa. Gene structure analysis indicated the differences existed in the gene structure of FAB1 family, the number of exons are 8-12. Conserve domain analysis showed that OsFAB1A and OsFAB1B contained the N-terminal FYVE domain, and the remaining members contained the Cpn60_TCP1 domain and PIPKc kinase domain. Phylogenetic analysis indicated that the functions of FAB1 family were highly conserved in mono- and dicotyledonous plants. Prediction of cis-element in the upstream regulatory region of FAB1 genes revealed a variety of growth-related, light-responsive, and hormone and stress-responsive cis-elements. Tissue expression profiles showed that most of FAB1 genes were global-expressed, and the high expression of FAB1C sub-cluster genes in lemma and palea suggested that they might be involved in floral organ development. Finally, the osfab1b mutants were obtained through the CRISPR/Cas9 system, and the pollen vitality of the osfab1b mutants showed no significantly abnormality, implied that FAB1 family had functional redundancy in the reproductive development of rice. The present results provide a theoretical reference for biological function studies of the phosphatidylinositol regulatory network in rice.


Rice (Oryza sativa); FAB1/PIKfyve gene family; Phosphatidylinositol 3,5-bisphosphate; CRISPR/Cas9
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