Research Report

Breeding of New Upland Rice Variety ‘Zhongkexilu 2’ by Molecular Marker-assisted Selection  

Liqiong Tang1 , Xiaoning Wang1 , Xinhua Wang1 , Xing Huo2 , Qingjie Tang1 , Jing Xu1 , Honglin Zhu1 , Peng Xu3 , Yanhui Lin1
1 Hainan Scientific Research Station of Crop Gene Resource & Germplasm Enhancement, Ministry of Agriculture, Hainan Key Laboratory of Crop Genetics and Breeding, Institute of Food Crops, Hainan Academy of Agricultural Sciences, Haikou, 571100, China
2 Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China
3 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Xishuangbanna, 666303, China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 20   doi: 10.5376/mpb.2022.13.0020
Received: 25 May, 2022    Accepted: 02 Jun., 2022    Published: 13 Jun., 2022
© 2022 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Tang L.Q., Wang X.N., Wang X.H., Huo X., Tang Q.J., Xu J., Zhu H.L., Xu P., Lin Y.H, 2022, Breeding of new upland rice variety ‘Zhongkexilu 2’ by molecular marker-assisted selection,Molecular Plant Breeding, 13(20): 1-7 (doi: 10.5376/mpb.2022.13.0020)


In order to cultivate new upland rice varieties with high yield and wide adaptability, which is suitable for Yunnan, Hainan and other tropical and subtropical areas, a new temperature sensitive upland rice variety ‘Zhongkexilu 2’ was cultivated by hybridization and backcrossing between rice variety ‘Minhui 63’ and upland rice variety ‘Lu 46’. The upland adaptive QTLs were introduced through the molecular marker assisted selection technology and pedigree method. The variety participated in evaluation trial at 3 sites in two years in Hainan. The average yield of multi-point regional trial in two years were 4 914 kg/hm2, which increased by more than 25.5% compared with the contrast variety 'Yunlu 201'. This shows that it has a strong potential for increasing production. The plant height was 105 cm, with panicle length of 22 cm. Effective panicles number per plant were 7, and grain number per panicle was 144. Seed setting rate was 89.2%, and the thousand grain weight was 24.1 g, showing the moderate plant type and panicle traits. The drought resistance of ‘Zhongkexilu 2’ was identified. The result showed that its drought resistance was basically the same as that of the upland parent ‘Luyin 46’, and it was significantly stronger than that of the parent ‘Minghui 63’. It was approved by Hainan Variety Certification Committee in 2019 (Qiongshen Rice 2018033). New varieties with high yield and drought resistant were cultivated through the molecular marker assisted selection technology and pedigree method, which was of great significance to promote the regeneration of upland rice varieties and the development of upland rice industry.

‘Zhongkexilu 2’; Upland rice; Molecular marker-assisted selection; Pedigree method
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