Analysis of Transcriptome Response to Low Temperature Stress in Mesembryanthemum crystallinum Linn.
Zhengfeng Lai *
Yunfa Yao *
Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, 363005, China
These authors contributed equally to this work
Molecular Plant Breeding, 2022, Vol. 13, No. 29 doi: 10.5376/mpb.2022.13.0029
Received: 15 Dec., 2022 Accepted: 20 Dec., 2022 Published: 25 Dec., 2022
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Lai Z.F., Yao Y.F., Lin B.Z., Lian D.M., and Hong J.J., 2022, Analysis of transcriptome response to low temperature stress in Mesembryanthemum crystallinum Linn., Molecular Plant Breeding, 13(29): 1-10 (doi: 10.5376/mpb.2022.13.0029)
The second generation of high throughput sequencing technology was used to sequence ice plant under low temperature stress and to construct transcriptome database. 24.13 Gb of valid data and 24 045 annotations of Unigene were obtained. DEGs of 1 902 (T0 vs T1) and 2 134 (T0 vs T2) were obtained. Two groups of DEGs were classified into 40 and 41 functional small categories respectively by GO database and were obtained respectively 20 and 24 functional categories by KOG database and were annotated 55 and 272 genes by comparing DEGs with KEGG database, which were enriched in 74 and 105 metabolic pathways respectively. DEGs of T0 vs T1 group mainly annotated to 4 metabolic pathways; DEGs of T0 vs T2 group mainly annotated to 11 metabolic pathways. It was showed that there were the metabolic pathways of positive correlation: acetone alcohol biosynthesis, purine metabolism, glutathione metabolism, fatty acid metabolism, brass metabolism, amino acid biosynthesis and other metabolic pathways, and the metabolic pathways of negative correlation: plant-pathogen interaction, plant hormone signal transduction, starch and sucrose metabolism. The key genes of starch and sucrose metabolism pathway were analyzed: the expression of 5 key genes, such as trehalose 6-phosphate synthase, trehalose-6-phosphatase, beta-amylase, glucose-1-adenosine phosphate transferase and glycogen phosphorylase, were up-regulated, but down-regulated gene was not observed during T1 (1h) of low-temperature stress; Three key genes of trehalose-6-phosphosynthase, hexokinase and beta-amylase, were up-regulated and the glucolactone-1,3-beta-glucosidase gene was down-regulated under low temperature stress at T2(36h). Eight DEGs in starch and sucrose metabolism pathway were selected and analyzed by Quantitative Real-time PCR. The relative expression level of 8 DEGs was consistent with the transcriptome expression profile analysis.
Ice plant; Transcriptome; DEGs; Starch and sucrose metabolism