Riyong Wang , Lingling Xie , Huoqiang Zhou , Yifei Wu , Wei Xiao , Zhuqing Zhang , Baobin Mi

Research Institute of Vegetables, Hunan Academy of Agricultural Sciences, Changsha, 410125, China
Author    Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 30   doi: 10.5376/mpb.2022.13.0030
Received: 15 Dec., 2022    Accepted: 22 Dec., 2022    Published: 27 Dec., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wang R.Y., Xie L.L., Zhou H.Q., Wu Y.F., Xiao W., Zhang Z.Q., and Mi B.B., 2022, Development and purity identification of InDel marker based on re-resequencing of “modilong” wax gourd, Molecular Plant Breeding, 13(30): 1-6 (doi: 10.5376/mpb.2022.13.0030)

In order to establish a simple, economic, accurate and reliable method to identify the purity of wax gourd hybrid seeds, an InDel marker characterized by significant differences was developed via whole genome re-sequencing of parent plants of ‘modilong’ wax gourd; Then the developed InDel molecular marker was used to identify the purity of hybrid seeds of ‘modilong’ taking the DNA of its hybrid seeds and parent plants as test DNA, and the obtained results were compared with field identification results. A total of 466 pairs of InDel markers were screened by genome re-resequencing of parent plants of ‘modilong’, and 26 of which with distinct differences were selected to be amplified and both got the strip. Among them, 7 pairs of primers can clearly distinguish the purity of wax gourd samples. The purity of InDel molecular marker identification was more than 99% consistent with the results of field plot planting identification, indicating that the purity results obtained by different methods were highly consistent. The InDel molecular marker screened in this study could be used as the purity identification of wax gourd hybrids.

Wax gourd; Seed purity; Indel molecular marker