Yanmei Wei¹ , Xinru Zhao¹ , Yujia Li¹ , Yuning Wang² , Jieqiu Li³ , Jiaorong Meng¹

1 College of Agriculture, Guangxi University, Nanning, 530005, China
2 College of Life Science and Technology, Guangxi University, Nanning, 530005, China
3 Agri-animal Industuial Development Institute, Guangxi University, Nanning, 530005, China
Author    Correspondence author
Molecular Plant Breeding, 2023, Vol. 14, No. 5   doi: 10.5376/mpb.2023.14.0005
Received: 14 Feb., 2023    Accepted: 21 Feb., 2023    Published: 28 Feb., 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wei Y.M., Zhao X.R., Li Y.J., Wang Y.N., Li J.Q., and Meng J.R., 2023, Cloning and expression analysis of MaMYB308 gene in mulberry, Molecular Plant Breeding, 14(5): 1-10 (doi: 10.5376/mpb.2023.14.0005)

In order to analyze the function of MYB transcription factors in mulberry abiotic stress and development, the full-length cDNA sequence of MYB transcription factor MaMYB308 was cloned from the mulberry cultivar ‘Guiyou 62’. The amino acid sequence of MYB transcription factor MaMYB308 was analyzed by bioinformatics, and its self activation was verified by yeast single hybridization. The expression pattern of the gene under different tissue and abiotic stresses was detected by real-time fluorescence quantitative PCR. The results showed that the full length of MaMYB308 gene is 1 266 bp, including an open reading frame of 1 026 bp, which can encode 341 amino acids, and its molecular weight is 39 kD; it has two SANT domains, belonging to typical R2R3 type MYB transcription factor, and has self activating activity. Blast results showed that MaMYB308 had high homology with a variety of R2R3-MYB transcription factors from higher plants, and had the highest homology with MYB308 from Morus notabilis, and the consistency of amino acid level was 96.48%. The results of real-time fluorescence quantitative PCR showed that the expression of MaMYB308 in roots, stems and leaves of mulberry was significantly higher than that in stems and leaves, and it was responsive to abiotic stresses such as high temperature, low temperature, high salt and drought. Among them, the expression of MaMYB308 was the most significant under low temperature stress, about 40 times of the control. The results of this study could be helpful for further study on the biological function of mulberry MaMYB308 gene and the stress resistance mechanism of mulberry.

Mulberry; R2R3 MYB transcription factor; Abiotic stress; Gene expression