Research Report

Appropriate Condition for Cluster Buds Induction of Cremastra appendiculata  

Sijia Liu , Yanyan Gao , Ningxian Yang , Li Tian , Sijing Peng , Mingsheng Zhang
School of Life Sciences, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, China
Author    Correspondence author
Molecular Plant Breeding, 2023, Vol. 14, No. 7   doi: 10.5376/mpb.2023.14.0007
Received: 21 Mar., 2023    Accepted: 26 Mar., 2023    Published: 05 Apr., 2023
© 2023 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Liu S.J., Gao Y.Y., Yang N.X., Tian L., Peng S.J., and Zhang M.S., 2023, Appropriate condition for cluster buds induction of Cremastra appendiculata, Molecular Plant Breeding, 14(7): 1-7 (doi: 10.5376/mpb.2023.14.0007)


To screen for suitable conditions for cluster bud induction of Cremastra appendiculata. The aseptic germination protocorms of C. appendiculata seeds were used as explant materials to explore the effects of different basic media, types of plant growth regulators, anti-browning agents and their concentrations on the induction of cluster buds of C. appendiculata. The results showed that: 1/4MS medium was the suitable basic medium for cluster buds growing; when the concentration of plant growth regulator TDZ was 2.0 mg/L and the concentration of IAA was 0.2 mg/L, the induction rates of cluster bud were higher which are 72.58% and 55.63% respectively; when the anti-browning agent was PVP and its concentration is 2 000 mg/L, the induction rate of cluster buds was higher which is 79.90%. The suitable conditions for C. appendiculata cluster buds induction are: 1/4MS + TDZ 2.0 mg/L+ IAA 0.1 mg/L+ PVP 2 000 mg/L, and the induction rate can reach 81.16%. This provided a basis for establishing a rapid propagation technology system of C. appendiculata and realizing its large-scale artificial cultivation.

Cremastra appendiculata; Cluster bud; Induction conditio
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