Jing Shang^1,2 , Xiaohui Liu^1,2 , Aidong Zhang² , Zongwen Zhu² , Dingshi Zha² , Lingjuan Kong³ , Xuexia Wu^1,2

1 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China
2 Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China
3 Shanghai Agricultural Technology Extension and Service Center, Shanghai, 201100, China
Author    Correspondence author
Molecular Plant Breeding, 2023, Vol. 14, No. 15   doi: 10.5376/mpb.2023.14.0015
Received: 15 May, 2023    Accepted: 12 Jun., 2023    Published: 11 Jul., 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Shang J., Liu X.H., Zhang A.D., Zhu Z.W., Zha D.S., Kong L.J., and Wu X.X., 2023, Cloning, bioinformatics and expression analysis of SmWRKY53 gene in Solanum melongena L., Molecular Plant Breeding, 14(15): 1-12 (doi: 10.5376/mpb.2023.14.0015)

In this study, SmWRKY53 gene was selected from the data of high temperture transcriptome and gene expression detection of "Tewangda" seedlings. It was then cloned, bioinformatics and preliminary expression analyzed. The results showed that the open reading frame of SmWRKY53 gene was 1 083 bp, encoding 360 amino acids. The 138-144 amino acids contain a typical "WRKYGQK" conserved domain, and the zinc finger structure is C₂HC type, belonging to the Group Ⅲ subfamily. The homology comparison and phylogenetic tree analysis showed that SmWRKY53 was closely related to StWRKY53 of potato (Solanum tuberosum), and the homology is 91.41%. Bioinformatics prediction showed that SmWRKY53 was a basic and unstable hydrophilic protein with no signal peptide and transmembrane domain. The ratio of random coil in the secondary structure of protein was the highest, 63.89%. There was amino acid disorder structural regions in the SmWRKY53 protein. Subcellular localization prediction indicated that the protein was most likely located in the nucleus. Quantitative Real-time PCR results showed that the relative expression levels of SmWRKY53 was the highest in mature stem and the lowest in flesh. The expression of SmWRKY53 in roots, stems and leaves of eggplant seedlings first increased and then decreased, and reached the peak at 3 h under high temperature stress. This study provides the reference for further exploring the function of SmWRKY53 gene.

Eggplant; SmWRKY53; Gene cloning; Bioinformatics; Expression analysis