Zhen Ying , Zhuang Zhou , Yanping Yang , Shuangbin Fu , Wan Xu

Zhejiang Institute of Subtropical Crops, Wenzhou, 325005, China
Author    Correspondence author
Molecular Plant Breeding, 2023, Vol. 14, No. 21   doi: 10.5376/mpb.2023.14.0021
Received: 09 Nov., 2023    Accepted: 14 Nov., 2023    Published: 23 Nov., 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Ying Z., Zhou Z., Yang Y.P., Fu S.B., and Xu W., 2023, Isolation of FLOWERING LOCUS T gene and analysis of periodic expression level in Cymbidium kanran, Molecular Plant Breeding, 14(21): 1-10 (doi: 10.5376/mpb.2023.14.0021)

Cymbidium kanran, as one of the five most famous Chinese Cymbidium, has high ornamental value, and it's principal flowering period varies from October to December. In order to study the flowering-regulation, the FLOWERING LOCUS T Gene (FT) of C. kanran sequence was amplified, and tissue-specific expression of the gene was analyzed in the whole flowering period. The results show: (1) The ORF sequence of the FT is 531 bp in length and the gene encodes 177 amino acids, and the sequence is highly homologous to the reported cymbidium such as C. ensifolium and C. goeringii; (2) During August to September, the expression of FT in the leaves of flowering C. kanran was 1.3 times higher than that of unflowered group, but there was no significant difference in the expression level of this gene at the other months; (3) During August to December, the expression level of FT in the flowering C. kanran pseudobulbs were 1.56, 2.46, 3.10, 2.70, and 1.42 times than that of unflowered group, respectively; (4) During flowering, the relative expression level of FT in pseudobulb of flowering C. kanran was at least 15.80 times higher than that in leaves; (5) The expression of FT in pseudobulbs were higher than that of flower stem and perianths, and slightly lower than that of primary flower bud. The above results fully show that there is an important correlation between the expression pattern of FT of the leaves and pseudobulbs in a specific period, which provides theoretical significance and application value for future research in the flowering-regulation of C. kanran and artificial flowering experiments.

Cymbidium kanran; Flowering period; FLOWERING LOCUS T gene; Expression patterns