Yiwei Li¹ , Wenbin Dong² , Liyu Liang¹ , Fuping Liu¹ , Hongli Li¹ , Huihong Liao¹ , Xi Wang¹

1 Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China
2 Agriculture Resource and Environment Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China
Author    Correspondence author
Molecular Plant Breeding, 2024, Vol. 15, No. 5   doi: 10.5376/mpb.2024.15.0021
Received: 07 Aug., 2024    Accepted: 10 Sep., 2024    Published: 18 Sep., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Li Y.W., Dong W.B., Liang L.Y., Liu F.P., Li H.L., Liao H.H., and Wang X., 2024, Application of multi-gene stacking strategies in citrus pest resistance breeding: from theory to practice, Molecular Plant Breeding, 15(5): 209-219 (doi: 10.5376/mpb.2024.15.0021)

Techniques such as gene aggregation, marker-assisted selection (MAS), and CRISPR-based genome editing have shown promising results in improving citrus resistance to pests and diseases. Gene polymerization has been successfully used to develop long-lasting resistant lines that are resistant to a variety of biological stresses, the combination of early-maturing transgenic plants with MAS has accelerated the breeding process and greatly reduced the time required to develop resistant varieties, and the CRISPR/Cas system has also played an important role in breeding citrus varieties that are resistant to various pathogens. The combination of advanced genetic engineering techniques and traditional breeding methods has the potential to produce robust citrus varieties that can withstand the challenges of multiple pests. Continued research and optimization of these strategies are essential for sustainable citrus production and improved crop resilience. This study highlights the significant advances in genetic engineering and biotechnology methods in the development of insect resistant citrus varieties, and aims to explore the application of polygenic stacking strategies in citrus pest resistance breeding, focusing on the theoretical basis and practical implementation of these strategies to improve the resistance of citrus to various pests.

Multi-gene stacking; Citrus pest resistance; Genetic engineering; Marker-assisted selection; CRISPR/Cas systems