Review Article
Application of Multi-Gene Stacking Strategies in Citrus Pest Resistance Breeding: From Theory to Practice
2 Agriculture Resource and Environment Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China
Author Correspondence author
Molecular Plant Breeding, 2024, Vol. 15, No. 5
Received: 07 Aug., 2024 Accepted: 10 Sep., 2024 Published: 18 Sep., 2024
Techniques such as gene aggregation, marker-assisted selection (MAS), and CRISPR-based genome editing have shown promising results in improving citrus resistance to pests and diseases. Gene polymerization has been successfully used to develop long-lasting resistant lines that are resistant to a variety of biological stresses, the combination of early-maturing transgenic plants with MAS has accelerated the breeding process and greatly reduced the time required to develop resistant varieties, and the CRISPR/Cas system has also played an important role in breeding citrus varieties that are resistant to various pathogens. The combination of advanced genetic engineering techniques and traditional breeding methods has the potential to produce robust citrus varieties that can withstand the challenges of multiple pests. Continued research and optimization of these strategies are essential for sustainable citrus production and improved crop resilience. This study highlights the significant advances in genetic engineering and biotechnology methods in the development of insect resistant citrus varieties, and aims to explore the application of polygenic stacking strategies in citrus pest resistance breeding, focusing on the theoretical basis and practical implementation of these strategies to improve the resistance of citrus to various pests.