Hongtao Gao , Haiyan Li

School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, 572025, Hainan, China
Author    Correspondence author
Molecular Plant Breeding, 2025, Vol. 16, No. 1   doi: 10.5376/mpb.2025.16.0004
Received: 20 Dec., 2024    Accepted: 23 Jan., 2025    Published: 31 Jan., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Gao H.T., and Li H.Y., 2025, Marker-assisted selection (MAS) in soybean breeding, Molecular Plant Breeding, 16(1): 35-43 (doi: 10.5376/mpb.2025.16.0004)

Marker-assisted selection (MAS) has become an indispensable tool in modern soybean breeding, enabling precise and efficient improvement of key agronomic traits. This study explores the principles and applications of MAS in enhancing both biotic and abiotic stress resistance, as well as quality and yield traits in soybean. The study begins by outlining the various genetic markers utilized in MAS, such as simple sequence repeats (SSRs), single nucleotide polymorphisms (SNPs), and quantitative trait loci (QTLs), along with the key techniques and tools employed, including high-throughput genotyping platforms, marker-assisted backcrossing (MABC), and genomic selection (GS). Following this, the study delves into the successful application of MAS in soybean trait improvement, providing an in-depth case study on soybean cyst nematode resistance, which exemplifies the effectiveness of MAS in addressing significant agricultural challenges. Recent technological advancements, such as the integration of MAS with genomic selection and the potential of CRISPR/Cas9 to complement MAS strategies, are discussed. The study also addresses current limitations, including cost, resource requirements, and genetic background effects, while providing insights into future directions that emphasize the integration of MAS with other emerging breeding technologies. Ultimately, this paper highlights the pivotal role of MAS in accelerating soybean breeding and its potential to contribute to the development of climate-resilient and high-yielding soybean varieties.

Marker-assisted selection; Soybean breeding; Genetic markers; Biotic and abiotic stress resistance; Trait improvement