Zhimin Dong , Shuming Wang , Jia Liu , Zhi Li , Zhigang Yi

Soybean Centre, Jilin Academy of Agriculture Science, Changchun, 130124, P.R. China
Author    Correspondence author
Molecular Plant Breeding, 2011, Vol. 2, No. 6   doi: 10.5376/mpb.2011.02.0006
Received: 29 Dec., 2010    Accepted: 25 Feb., 2011    Published: 15 Apr., 2011

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Dong et al., 2011, Preliminary mapping of soybean dominant locus Hrcs7 conferring resistance to Cerocospara sojina race 7, Molecular Plant Breeding Vol.2 No.6 (doi: 10.5376/mpb.2011.02.0006)

Molecular marker-assisted selection can significantly improved resistance breeding efficiency. The soybean frogeye leaf spot (FLS) is caused by the fungus Cercospora sojina Hara. In order to find the resistant markers linked to C. sojina race 7 in soybean, we carried out the resistant genetic ananlysis and the resistant locus mapping. The F₂ population derived from the cross of 'Gang 95144-1' and 'Gongjiao 9723-6' was employed to analyze the resistance genetics. it was sure that the resistance conferring to FLS was controlled by one major dominant gene.. Bulk Segregant Analysis (BSA) and Simple Sequence Repeat (SSR) method were used to map the resistant locus. Among the 600 selected SSR primers, there were four primer pairs exhibiting the polymorphisms between DNA bulk of resistant and susceptible as well as both parents. Segregation distribution of the SSR markers, satt 414 and satt384, were conformed significantly to be as 1:2:1.of Mendel Segregation. The marker, satt411 and satt384 were assigned to link with the resistant locus based on estimation of MAPMAKER/EXP 3.0 software. The linkage order and distance would be Satt384 -5.7cM - Satt411 -7.9cM -Hrcs7. The resistant locus was integrated to soybean linkage group E based on the Cregen^'s genetic linkage map of soybean.Molecular marker-assisted selection can significantly improved resistance breeding efficiency. The soybean frogeye leaf spot (FLS) is caused by the fungus Cercospora sojina Hara. In order to find the resistant markers linked to C. sojina race 7 in soybean, we carried out the resistant genetic ananlysis and the resistant locus mapping. The F₂ population derived from the cross of 'Gang 95144-1' and 'Gongjiao 9723-6' was employed to analyze the resistance genetics. it was sure that the resistance conferring to FLS was controlled by one major dominant locus named Hrcs7.. Bulk Segregant Analysis (BSA) and Simple Sequence Repeat (SSR) method were used to map the resistant locus. Among the 600 selected SSR primers, there were four primer pairs exhibiting the polymorphisms between DNA bulk of resistant and susceptible as well as both parents. Segregation distribution of the SSR markers, satt 414 and satt384,  were conformed significantly to be as 1:2:1.of Mendel Segregation. The marker, satt411 and satt384,  were assigned to link with the resistant locus based on estimation of MAPMAKER/EXP 3.0 software.  The linkage order and distance would be Satt384 -5.7cM -Satt411 -7.9cM -Hrcs7. The resistant locus was integrated to soybean linkage group E based on the Cregen^'s genetic linkage map of soybean.

Soybean (Glycine max); Cercospora leaf spot Race 7; Resistant locus; Hrcs7; Genetic mapping