In this study, the whole genome DNA of sorghum apomixes line SSA-1 (Shanxi sorghum apomict-1) was injected into two rice varieties of ‘Liaoyan28’ and ‘Liaoyan6’ using the pollen tube pathway method. The genetic characters of the progeny changed obviously. By self-selection and identification, 27 phenotypic stable D₄ generation lines were obtained. Through field planting, the phenotypes of agronomic traits such as heading date, tiller number, plant height, and 1 000-grain weight of the introduced lines were statistically analyzed. The results showed that the agronomic traits of the D₄ generation lines were widely varied, some D₄ generation lines were more inclined to donor sorghum in culm and spike color, which indicated that the whole genome DNA of sorghum was successfully introduced into the receptor cells, and the expression of the receptor gene was affected. Compared with the recipient parents, the introduced lines showed significant variations in the main traits such as heading date, tiller number, plant height, grain number per ear, grain weight per ear, ear length, and thousand grain weights. Further analysis and selection of mutant lines is helpful to discover new germplasm materials and provide abundant germplasm resources for breeding new rice varieties.